Recombinant Anti-XAB2 antibody [EPR8971] (ab180181)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8971] to XAB2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-XAB2 antibody [EPR8971]
See all XAB2 primary antibodies -
Description
Rabbit monoclonal [EPR8971] to XAB2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human XAB2 aa 750-850 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HCS7 -
Positive control
- HeLa, K562 and HepG2 whole cell lysate (ab7900); Human breast and Human uterus tissues; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8971 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing. -
Sequence similarities
Belongs to the crooked-neck family.
Contains 14 HAT repeats. -
Cellular localization
Nucleus. Detected in the splicing complex carrying pre-mRNA. - Information by UniProt
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Database links
- Entrez Gene: 56949 Human
- Omim: 610850 Human
- SwissProt: Q9HCS7 Human
- Unigene: 9822 Human
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Alternative names
- 0610041O14Rik antibody
- adapter protein ATH 55 antibody
- Adapter protein ATH-55 antibody
see all
Images
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All lanes : Anti-XAB2 antibody [EPR8971] (ab180181) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa -
Immunofluorescent analysis of HepG2 cells labeling XAB2 with ab180181 at 1/50.
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Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling XAB2 with ab180181 at 1/50.
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Immunohistochemical analysis of paraffin embedded Human breast tissue labeling XAB2 with ab180181 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab180181 has not yet been referenced specifically in any publications.