Overview
Fluorescent A3 antagonist
A3-633-ANXanthine Amine Congener (XAC)-derivative
Excitation wavelength: 633 nm
Emission wavelength: 650 nm
Properties
To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at A1, A2A and A3 receptors was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
Applications
Our Abpromise guarantee covers the use of ab118164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Small Molecule Fluorescent Optical Detection | Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration. |
| Receptor Localization | Receptor Localization: Use at an assay dependent concentration. |
| Fluorescent Cell Imaging | Fluorescent Cell Imaging: Use at an assay dependent concentration. |
XAC Fluorescent ligand (Red) images
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Receptor Localization - XAC Fluorescent ligand-2 (Red) (ab118164)Left: ab118164 ligand (100 nM) binding to live CHO cells expressing adenosine A3 receptors.
Right: Binding blocked by the unlabelled competitor XAC (10 µM). Nuclei have been counter-stained with Hoechst. -
Small Molecule Fluorescent Optical Detection - XAC Fluorescent ligand-2 (Red) (ab118164)To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at A1 receptor was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
Antagonist -log KD values for A1 is 6.57. -
Small Molecule Fluorescent Optical Detection - XAC Fluorescent ligand-2 (Red) (ab118164)To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at A2A receptor was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
the Antagonist -log KD values for A2A is 6.74. -
Small Molecule Fluorescent Optical Detection - XAC Fluorescent ligand-2 (Red) (ab118164)To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
The apparent KD at A3 receptors was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
The Antagonist -log KD value for A3 is 8.1.
References for XAC Fluorescent ligand (Red) (ab118164)
ab118164 has not yet been referenced specifically in any publications.
