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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab17204? Please let us know so that we can cite the reference in this datasheet
ab17204 has been referenced in 2 publications.
- Wang Y et al. Targeted proteasome inhibition by Velcade induces apoptosis in human mesothelioma and breast cancer cell lines. Cancer Chemother Pharmacol 66:455-66 (2010). WB; Human. PubMed: 19960346
- Russell JC et al. Nuclear translocation of X-linked inhibitor of apoptosis (XIAP) determines cell fate after hypoxia ischemia in neonatal brain. J Neurochem 106:1357-70 (2008). ICC/IF; Rat. PubMed: 18485100
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - XAF1 antibody (ab17204)
Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lane 2 : Human spleen lysate
Lane 3 : Human spleen lysate
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XAF1 antibody (ab17204)
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
Immunocytochemistry/ Immunofluorescence-XAF1 antibody(ab17204)
ICC/IF image of ab17204 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17204, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
XAF1 antibody for WB in Rat (17204)
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