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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Overview
Product name
Anti-XAF1 antibody
See all XAF1 products (7) ...
Description
Rabbit polyclonal to XAF1
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).
Positive control
Human spleen cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18485100)
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 32 kDa.Can be blocked with XAF1 peptide (305-317) (ab39906). (A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.)
IHC-P: Use a concentration of 2 µg/ml
Target
Function
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Tissue specificity
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Sequence similarities
Contains 1 TRAF-type zinc finger.
Developmental stage
Selectively expressed in third trimester placenta.
Cellular localization
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
Target information above from: UniProt accessionQ6GPH4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
see all
Database links
- Entrez Gene: 54739 Human
- Entrez Gene: 327959 Mouse
- Entrez Gene: 679600 Rat
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- SwissProt: Q5NBU8 Mouse
- Unigene: 441975 Human
- Unigene: 291131 Mouse
Anti-XAF1 antibody images:
Western blot - XAF1 antibody (ab17204)
Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lane 2 : Human spleen lysate
Lane 3 : Human spleen lysate
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XAF1 antibody (ab17204)
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
Immunocytochemistry/ Immunofluorescence-XAF1 antibody(ab17204)
ICC/IF image of ab17204 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-XAF1 antibody (ab17204)
This product has been referenced in:
- Wang Yet al. Targeted proteasome inhibition by Velcade induces apoptosis in human mesothelioma and breast cancer cell lines. Cancer Chemother Pharmacol 66:455-66 (2010). WB; Human.Read more (PubMed: 19960346) »
- Russell JCet al. Nuclear translocation of X-linked inhibitor of apoptosis (XIAP) determines cell fate after hypoxia ischemia in neonatal brain. J Neurochem 106:1357-70 (2008). ICC/IF; Rat.Read more (PubMed: 18485100) »
See all 2 publications for this product
Publishing research using ab17204? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"