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Western blot - XAF1 antibody (ab25889)
(enlarge)
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Product Name
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XAF1 antibody
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See all XAF1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XAF1
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Immunogen
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Synthetic peptide (Human) - corresponding to 16 amino acids near the middle of human XAF-1.
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Reacts with
(species key)
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Hu
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Specificity
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ab25889 recognises XAF-1.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-2 µg/ml. Predicted molecular weight: 34 kDa.
Can be blocked with XAF1 peptide (ab39908).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human spleen cell lysate.
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Cellular localization
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Cytoplasmic, Mitochondrial and Nuclear
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Research areas
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Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
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Relevance
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XAF1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF1 results in the neutralization of XIAP's ability to inhibit cell death. XAF1 is normally expressed in all adult and fetal tissues but is present at very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XAF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XAF1 antibody images, references, products related to ab25889 and other tools.
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XAF1 antibody images:
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 Western blot - XAF1 antibody (ab25889)
Lane 1 : XAF1 antibody (ab25889) at 1 µg/ml
Lane 2 : XAF1 antibody (ab25889) at 2 µg/ml
Lane 3 : XAF1 antibody (ab25889) at 4 µg/ml
Lane 1 : Human spleen cell lysate.
Lane 2 : Human spleen cell lysate.
Lane 3 : Human spleen cell lysate.
Predicted band size : 34 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
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References for XAF1 antibody (ab25889)
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This product has been used in:
Bai Y et al. Interferon-gamma induces XIAP-associated factor-1 and NOXA expression and potentiates human vascular smooth muscle cell apoptosis by STAT3 activation. J Biol Chem : (2008). WB; Human. PubMed: 18192275
If you publish research using ab25889 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XAF1
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XAF1 antibody - more information
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Customer reviews (feedback) regarding XAF1 antibody |
Customer FAQs regarding XAF1 antibody |
Protocols for XAF1 antibody |
Price and availability of products related to XAF1 antibody |
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XAF1 antibody - related products:
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XAF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XAF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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