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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Overview
Product name
Anti-XAF1 antibody
See all XAF1 products (7) ...
Description
Rabbit polyclonal to XAF1
Specificity
ab25889 recognises XAF-1.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) - corresponding to 16 amino acids near the middle of human XAF-1.
Positive control
Human spleen cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
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Western blot - XAF1 antibody (ab25889)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1-2 µg/mlPredicted molecular weight: 34 kDa.Can be blocked with XAF1 peptide (ab39908).
Target
Function
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Tissue specificity
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Sequence similarities
Contains 1 TRAF-type zinc finger.
Developmental stage
Selectively expressed in third trimester placenta.
Cellular localization
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
Target information above from: UniProt accessionQ6GPH4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
see all
Database links
- Entrez Gene: 54739 Human
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- Unigene: 441975 Human
Anti-XAF1 antibody images:
Western blot - XAF1 antibody (ab25889)
Lane 1 : Anti-XAF1 antibody (ab25889) at 1 µg/ml
Lane 2 : Anti-XAF1 antibody (ab25889) at 2 µg/ml
Lane 3 : Anti-XAF1 antibody (ab25889) at 4 µg/ml
Lane 1 : Human spleen cell lysate.
Lane 2 : Human spleen cell lysate.
Lane 3 : Human spleen cell lysate.
Predicted band size : 34 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-XAF1 antibody (ab25889)
This product has been referenced in:
- Desplanques Get al. Impact of XIAP protein levels on the survival of myeloma cells. Haematologica 94:87-93 (2009). WB; Human.Read more (PubMed: 19001278) »
- Bai Yet al. Interferon-gamma induces XIAP-associated factor-1 and NOXA expression and potentiates human vascular smooth muscle cell apoptosis by STAT3 activation. J Biol Chem : (2008). WB; Human.Read more (PubMed: 18192275) »
See all 2 publications for this product
Publishing research using ab25889? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"