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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Overview
Product name
XAF1 peptide
See all XAF1 products (3) ...
Protein description
Synthetic peptide, corresponding to amino acids from near the middle of Human XAF1.
Properties
Purification notes
ab39908 is a crude synthetic peptide, purity range from 60-70%. The sequence is proprietary.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Blocking
Application notes
BL: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
This peptide can be used with studies using ab25889.
It usually blocks the antibody activity completely in immunoblot analysis by incubating the peptide with equal volume of antibody for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Tissue specificity
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Sequence similarities
Contains 1 TRAF-type zinc finger.
Developmental stage
Selectively expressed in third trimester placenta.
Cellular localization
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
Target information above from: UniProt accessionQ6GPH4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for XAF1 peptide (ab39908)
ab39908 has not yet been referenced specifically in any publications.
Publishing research using ab39908? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"