Overview
Product nameXAF1 peptideSee all XAF1 proteins and peptides ...
Protein descriptionSynthetic peptide, corresponding to amino acids from near the middle of Human XAF1.
Properties
Purification notesab39908 is a crude synthetic peptide, purity range from 60-70%. The sequence is proprietary.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS, pH 7.2
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab39908
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| WB |
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| Blocking |
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Application notesBL: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
This peptide can be used with studies using ab25889.
It usually blocks the antibody activity completely in immunoblot analysis by incubating the peptide with equal volume of antibody for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
BIRC 4 binding proteinBIRC4 binding proteinBIRC4-binding protein
BIRC4BPHSXIAPAF 1HSXIAPAF1XAF 1XAF1XAF1_HUMANXIAP associated factor 1XIAP-associated factor 1XIAPAF1
see all
FunctionSeems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Tissue specificityWidely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Sequence similaritiesContains 1 TRAF-type zinc finger.
Developmental stageSelectively expressed in third trimester placenta.
Cellular localizationNucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
References for XAF1 peptide (ab39908)
ab39908
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
