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Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-XAGE2 antibody
Description
Mouse polyclonal to XAGE2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human XAGE2 protein (NP_570133.1, 1 a.a. ~ 111 a.a)
Positive control
XAGE2 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).
Target
Relevance
XAGE2 belongs to the GAGE family of genes which are expressed in a variety of tumors and in some fetal and reproductive tissues. XAGE2 is strongly expressed in normal testis, and in Ewing's sarcoma, rhabdomyosarcoma, a breast cancer and a germ cell tumor. Its function is unknown.
Alternative names
- Cancer/testis antigen 12.2 antibody
- CT12.2 antibody
- G antigen family D 3 antibody
see all
Database links
- Entrez Gene: 9502 Human
- Omim: 300416 Human
- SwissProt: Q96GT9 Human
Anti-XAGE2 antibody images:
Western blot - XAGE2 antibody (ab69890)
All lanes : Anti-XAGE2 antibody (ab69890) at 1/500 dilution
Lane 1 : XAGE2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 12 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - XAGE2 antibody (ab69890)
ab69890 at 10µg/ml staining XAGE2 Hela cells by Immunocytochemistry/Immunofluorescence.
References for Anti-XAGE2 antibody (ab69890)
ab69890 has not yet been referenced specifically in any publications.
Publishing research using ab69890? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"