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ab30769 |
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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-XBP1 antibody
See all XBP1 products (11) ...
Rabbit polyclonal to XBP1
Reacts with
Rat, Human
Predicted to work with
Mouse
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human XBP1.
(Peptide available as ab30769.)
This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, A431 Whole Cell, Rat Testis Tissue
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot - XBP1 antibody (ab28715)
(enlarge)
Our Abpromise guarantee covers the use of ab28715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). We believe this band represents the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
Belongs to the bZIP family.
Contains 1 bZIP domain.
Nucleus.
Target information above from: UniProt accessionP17861
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - XBP1 antibody (ab28715)

All lanes : Anti-XBP1 antibody (ab28715) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Testis (Rat) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
We believe the 35 kDa band corresponds to the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.
ab28715 has not yet been referenced specifically in any publications.
Publishing research using ab28715? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-XBP1 antibody (ab28715) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Testis (Rat) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
We believe the 35 kDa band corresponds to the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.
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