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ab39909 |
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ab37151 has been referenced in 2 publications.
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Predicted band size : 29 kDa
Western blot analysis of XBP-1 in PC-3 cell lysate with ab37151 at (A) 0.5, (B) 1 and (C) 2 µg/ml.
All lanes : Anti-XBP1 antibody (ab37151) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 29 kDa,86 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to XBP1.
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