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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-XBP1 antibody
See all XBP1 products (11) ...
Description
Rabbit polyclonal to XBP1
Specificity
This product will recognize only the non-spliced (shorter) form of XBP1.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to 17 amino acids near to the C terminus of the human XBP1 protein.
Positive control
PC3 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 2 µg/ml
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).Can be blocked with XBP1 peptide (ab39909).
Target
Function
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
Involvement in disease
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP17861
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- XBP2 antibody
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
see all
Database links
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all
Anti-XBP1 antibody images:
- XBP1 antibody (ab37151)
Western blot analysis of XBP-1 in PC-3 cell lysate with ab37151 at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Western blot - XBP1 antibody (ab37151)
All lanes : Anti-XBP1 antibody (ab37151) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 29 kDa,86 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to XBP1.
References for Anti-XBP1 antibody (ab37151)
This product has been referenced in:
- Gavilán MPet al. Dysfunction of the unfolded protein response increases neurodegeneration in aged rat hippocampus following proteasome inhibition. Aging Cell 8:654-65 (2009). WB; Rat.Read more (PubMed: 19747230) »
See 1 publication for this product
Publishing research using ab37151? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"