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Western blot - XBP1 antibody (ab37152)
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Immunocytochemistry - XBP1 antibody (ab37152)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)
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Product Name
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XBP1 antibody
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See all XBP1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XBP1
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Immunogen
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Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at an assay dependent dilution (PMID 19543371).
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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L1210 cell lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XBP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XBP1 antibody images, references, products related to ab37152 and other tools.
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XBP1 antibody images:
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 Western blot - XBP1 antibody (ab37152)
Lane 1 : XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : XBP1 antibody (ab37152) at 4 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
goat anti-rabbit HRP at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the XBP1 band.
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Immunocytochemistry - XBP1 antibody (ab37152)
Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)
ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin. Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).
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References for XBP1 antibody (ab37152)
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This product has been used in: (two most recent references)
Ren Y et al. Genetic variation of promoter sequence modulates XBP1 expression and genetic risk for vitiligo. PLoS Genet 5:e1000523 (2009). IHC-P; Human. PubMed: 19543371
Hsiao JR et al. Endoplasmic reticulum stress triggers XBP-1-mediated up-regulation of an EBV oncoprotein in nasopharyngeal carcinoma. Cancer Res 69:4461-7 (2009). IHC-P; Human. PubMed: 19435892
See all 4 publication references for this product.
If you publish research using ab37152 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XBP1
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XBP1 antibody - more information
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Customer reviews (feedback) regarding XBP1 antibody |
Customer FAQs regarding XBP1 antibody |
Protocols for XBP1 antibody |
Price and availability of products related to XBP1 antibody |
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XBP1 antibody - related products:
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XBP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XBP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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