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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-XBP1 antibody
See all XBP1 products (11) ...
Description
Rabbit polyclonal to XBP1
Specificity
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
Tested applications
IHC-P, IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein.
Positive control
L1210 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20028872Use at an assay dependent dilution (PMID 20028872). )
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 29 kDa.Can be blocked with XBP1 peptide (ab39910). (Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). )
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
Involvement in disease
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP17861
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- XBP2 antibody
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
see all
Database links
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- Unigene: 437638 Human
- Unigene: 469937 Mouse
Anti-XBP1 antibody images:
Western blot - XBP1 antibody (ab37152)
Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
goat anti-rabbit HRP at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the XBP1 band.
Immunocytochemistry - XBP1 antibody (ab37152)
Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 µg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)
ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)
ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
Image courtesy of an anonymous Abreview.
References for Anti-XBP1 antibody (ab37152)
This product has been referenced in:
- Chang KCet al. Dominant expression of survival signals of endoplasmic reticulum stress response in Hodgkin lymphoma. Cancer Sci 102:275-81 (2011). WB; Human.Read more (PubMed: 21062387) »
- Spiotto MTet al. Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth. Cancer Res 70:78-88 (2010). IHC-Fr; Human.Read more (PubMed: 20028872) »
See all 6 publications for this product
Publishing research using ab37152? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"