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ab39910 |
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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-XBP1 antibody
See all XBP1 products (11) ...
Rabbit polyclonal to XBP1
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
IHC-P, IHC-Fr, WB, ICC/IFmore details
Reacts with
Mouse, Human
Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein.
L1210 cell lysate
Liquid
Store at +4°C.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20028872Use at an assay dependent dilution (PMID 20028872). )
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 29 kDa.Can be blocked with XBP1 peptide (ab39910). (Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). )
ICC/IF: Use a concentration of 10 µg/ml
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
Belongs to the bZIP family.
Contains 1 bZIP domain.
Nucleus.
Target information above from: UniProt accessionP17861
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - XBP1 antibody (ab37152)

Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
goat anti-rabbit HRP at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the XBP1 band.
Immunocytochemistry - XBP1 antibody (ab37152)

Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 µg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)

ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody (ab37152)

ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See all 6 publications for this product
Publishing research using ab37152? Please let us know so that we can cite the reference in this datasheet
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Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
goat anti-rabbit HRP at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the XBP1 band.

Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 µg/ml

ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).

ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
Image courtesy of an anonymous Abreview.

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