XIAP antibody [2F1] (ab28151)

Product Name 

XIAP antibody [2F1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2F1] to XIAP

Immunogen 

Fusion protein with Human XIAP.

Reacts with 
(species key)

Hu, Ms

Epitope 

ab28151 reacts with an epitope located in the C terminal region encoded by aa 352/449 of XIAP.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa Cell Lysate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs

Relevance 

The human X chromosome linked Inhibitor of Apoptosis Protein (XIAP) is a major member of the inhibitor of apoptosis (IAP) family. XIAP functions by direct binding and inhibition of several caspases, including Caspase 3, Caspase 7 and Caspase 9. During apoptosis XIAP is specifically cleaved by Caspase 3 and partially loses its function. In addition to blocking the apoptosis signaling pathway, XIAP can also bind several other proteins in the bone morphogenetic protein (BMP) signaling pathway and act as a coupler between the membrane BMP receptor and the TAB1 TAK1 complex by binding to these proteins.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2F1

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for XIAP antibody [2F1] (ab28151)

  This product has been used in:

Jost PJ et al. XIAP discriminates between type I and type II FAS-induced apoptosis. Nature 460:1035-9 (2009). WB, IP; Mouse. PubMed: 19626005

If you publish research using ab28151 please let us know so that we can cite the reference on this datasheet.

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