 image](#XIAP-Primary-antibodies-ab28151-3.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-XIAP antibody [2F1]
See all XIAP products (9) ...
Description
Mouse monoclonal [2F1] to XIAP
Tested applications
Flow Cyt, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Fusion protein with Human XIAP.
Epitope
ab28151 reacts with an epitope located in the C terminal region encoded by aa 352/449 of XIAP.
Positive control
HeLa Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
2F1
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
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Flow Cytometry-XIAP antibody [2F1](ab28151)
 image](/ps/datasheet/images/28/ab28151/XIAP-Primary-antibodies-ab28151-3.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 2µg for 106 cells.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 55 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
Tissue specificity
Ubiquitous, except peripheral blood leukocytes.
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
Post-translational
modifications
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP98170
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AP 13 antibody
- API3 antibody
- API3 antibody
see all
Database links
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
- Unigene: 356076 Human
- Unigene: 259879 Mouse
Anti-XIAP antibody [2F1] images:
Flow Cytometry-XIAP antibody [2F1](ab28151)
Overlay histogram showing HeLA cells stained with ab28151 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28151, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-XIAP antibody [2F1] (ab28151)
This product has been referenced in:
- Jost PJet al. XIAP discriminates between type I and type II FAS-induced apoptosis. Nature 460:1035-9 (2009). WB, IP; Mouse.Read more (PubMed: 19626005) »
- Ali Set al. The development of a selective cyclin-dependent kinase inhibitor that shows antitumor activity. Cancer Res 69:6208-15 (2009). WB; Human.Read more (PubMed: 19638587) »
See all 3 publications for this product
Publishing research using ab28151? Please let us know so that we can cite the reference in this datasheet
![XIAP antibody [2F1] for WB in Mouse (28151)](/ps/datasheet/images/28/ab28151/XIAP-Primary-antibodies-ab28151-2.png)
![Anti-XIAP antibody [2F1] for Western blot in Human (28151)](/ps/datasheet/images/28/ab28151/XIAP-Primary-antibodies-ab28151-8.jpg)
![XIAP antibody [2F1] for WB in Human (28151)](/ps/Reviews/Images/ab8984_43268.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"