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ab21278 has been referenced in 7 publications.
Publishing research using ab21278? Please let us know so that we can cite the reference in this datasheet
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Lane 1 : Anti-XIAP antibody (ab21278) at 0.5 µg/ml
Lane 2 : Anti-XIAP antibody (ab21278) at 1 µg/ml
Lane 3 : Anti-XIAP antibody (ab21278) at 2 µg/ml
Lane 1 : Human kidney lysate
Lane 2 : Human kidney lysate
Lane 3 : Human kidney lysate
Predicted band size : 55 kDa
ab21278 at 2µg/ml staining XIAP in human kidney tissue by IHC
ICC/IF image of ab21278 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21278, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-XIAP antibody (ab21278) at 2 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
3
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