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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-XIAP antibody
See all XIAP products (9) ...
Description
Rabbit polyclonal to XIAP
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP.
Positive control
Human kidney cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Purified by ion exchange chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.5 - 2 µg/ml.Predicted molecular weight: 55 kDa.
Target
Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
Tissue specificity
Ubiquitous, except peripheral blood leukocytes.
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
Post-translational
modifications
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP98170
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AP 13 antibody
- API3 antibody
- API3 antibody
see all
Database links
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
- Unigene: 356076 Human
- Unigene: 259879 Mouse
Anti-XIAP antibody images:
Western blot - XIAP antibody (ab21278)
Lane 1 : Anti-XIAP antibody (ab21278) at 0.5 µg/ml
Lane 2 : Anti-XIAP antibody (ab21278) at 1 µg/ml
Lane 3 : Anti-XIAP antibody (ab21278) at 2 µg/ml
Lane 1 : Human kidney lysate
Lane 2 : Human kidney lysate
Lane 3 : Human kidney lysate
Predicted band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XIAP antibody (ab21278)
ab21278 at 2µg/ml staining XIAP in human kidney tissue by IHC
Immunocytochemistry/ Immunofluorescence-XIAP antibody(ab21278)
ICC/IF image of ab21278 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21278, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-XIAP antibody (ab21278)
This product has been referenced in:
- Kim SHet al. Garlic constituent diallyl trisulfide suppresses x-linked inhibitor of apoptosis protein in prostate cancer cells in culture and in vivo. Cancer Prev Res (Phila) 4:897-906 (2011).Read more (PubMed: 21411500) »
- Courter Det al. The RGD domain of human osteopontin promotes tumor growth and metastasis through activation of survival pathways. PLoS One 5:e9633 (2010). WB; Human.Read more (PubMed: 20224789) »
See all 7 publications for this product
Publishing research using ab21278? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"