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Immunocytochemistry/ Immunofluorescence - XIAP antibody (ab2541)
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Product Name
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XIAP antibody
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See all XIAP antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XIAP
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Immunogen
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Available on request.
(Peptide available as ab38275.)
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Reacts with
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Hu, Ms, Rat
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Specificity
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This antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors.
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Tested applications
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ICC/IF, IHC (PFA fixed), WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/200.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution (See PMID: 17094439).
WB: Use at a concentration of 1.0 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
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Relevance
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The human X chromosome linked Inhibitor of Apoptosis Protein (XIAP) is a major member of the inhibitor of apoptosis (IAP) family. XIAP functions by direct binding and inhibition of several caspases, including Caspase 3, Caspase 7 and Caspase 9. During apoptosis XIAP is specifically cleaved by Caspase 3 and partially loses its function. In addition to blocking the apoptosis signaling pathway, XIAP can also bind several other proteins in the bone morphogenetic protein (BMP) signaling pathway and act as a coupler between the membrane BMP receptor and the TAB1 TAK1 complex by binding to these proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XIAP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XIAP antibody images, references, products related to ab2541 and other tools.
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XIAP antibody images:
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 Immunocytochemistry/ Immunofluorescence - XIAP antibody (ab2541)
ab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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References for XIAP antibody (ab2541)
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This product has been used in: (two most recent references)
Lopergolo A et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB; Human. PubMed: 19223905
Shrikhande SV et al. Silencing of X-linked inhibitor of apoptosis (XIAP) decreases gemcitabine resistance of pancreatic cancer cells. Anticancer Res 26:3265-73 (2006). Immunohistochemistry (PFA fixed). PubMed: 17094439
If you publish research using ab2541 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XIAP
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XIAP antibody - more information
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Customer reviews (feedback) regarding XIAP antibody |
Customer FAQs regarding XIAP antibody |
Protocols for XIAP antibody |
Price and availability of products related to XIAP antibody |
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XIAP antibody - related products:
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XIAP antibody - other tools:
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Contact Abcam with a Technical Enquiry about XIAP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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