Anti-XIAP antibody (ab59282)

Overview

• Product nameAnti-XIAP antibodySee all XIAP primary antibodies ...
• Description
  Rabbit polyclonal to XIAP
• Specificityab59282 detects endogenous levels of total XIAP protein.
• Tested applicationsIHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  A synthesized non phosphopeptide derived from human XIAP around the phosphorylation site of serine 87 (KVS^PPN).

• Positive controlHuman skeletal muscle tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Caspases

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Death receptors & ligands
  • IAPs

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Survivin / IAPs

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Inhibitors

Applications

Target

• FunctionApoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
• Tissue specificityUbiquitous, except peripheral blood leukocytes.
• Involvement in diseaseDefects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
• Sequence similaritiesBelongs to the IAP family.
  Contains 3 BIR repeats.
  Contains 1 RING-type zinc finger.
• DomainThe first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
• Post-translational
  modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
  Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P98170 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 331 Human
  • Entrez Gene: 331 Human
  • Entrez Gene: 11798 Mouse
  • Entrez Gene: 63879 Rat
  • Omim: 300079 Human
  • SwissProt: P98170 Human
  • SwissProt: P98170 Human
  • SwissProt: Q60989 Mouse

  • SwissProt: Q9R0I6 Rat
  • Unigene: 356076 Human
  • Unigene: 259879 Mouse
  • Unigene: 91239 Rat

  see all

• Alternative names
  AP 13 antibodyAPI3 antibodyApoptosis Inhibitor 3 antibody

  Baculoviral IAP repeat containing 4 antibodyBaculoviral IAP Repeat Containing Protein 4 antibodyBaculoviral IAP repeat-containing protein 4 antibodyBIRC 4 antibodyBIRC4 antibodyE3 ubiquitin-protein ligase XIAP antibodyhIAP-3 antibodyhIAP3 antibodyHILP antibodyIAP 3 antibodyIAP like protein antibodyIAP-3 antibodyIAP-like protein antibodyIAP3 antibodyILP 1 antibodyILP antibodyILP1 antibodyInhibitor of apoptosis protein 3 antibodyInhibitor of Apoptosis X Linked antibodyMammalian IAP Homologue A antibodyMIHA antibodyX linked IAP antibodyX linked inhibitor of apoptosis antibodyX linked inhibitor of apoptosis protein antibodyX-linked IAP antibodyX-linked inhibitor of apoptosis protein antibodyXiap antibodyXIAP_HUMAN antibodyXLP2 antibody

  see all

Anti-XIAP antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - XIAP antibody (ab59282)
  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab59282 at a dilution of 1/50. Left hand image - without immunizing peptide; right hand image - with immunizing peptide.