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Immunohistochemistry (Paraffin-embedded sections) - XIAP (phospho S87) antibody (ab59289)
Ab59289 at 1/50 dilution staining human skeletal muscle without (left) and with (right) immunizing peptide; paraffin embedded.
Western blot - XIAP (phospho S87) antibody (ab59289)
Anti-XIAP (phospho S87) antibody (ab59289) at 1 µg/ml + T24/83 (Human bladder carcinoma) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 55 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
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