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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-XIAP (phospho S87) antibody
See all XIAP products (9) ...
Description
Rabbit polyclonal to XIAP (phospho S87)
Specificity
Detects endogenous levels of XIAP only when phosphorylated at serine 87.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human XIAP around the phosphorylation site of serine 87 (K-V-SP-P-N).
Positive control
Human skeletal muscle.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 51 kDa (predicted molecular weight: 55 kDa).
Target
Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
Tissue specificity
Ubiquitous, except peripheral blood leukocytes.
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
Post-translational
modifications
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP98170
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
Database links
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Entrez Gene: 63879 Rat
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
- SwissProt: Q9R0I6 Rat
- Unigene: 356076 Human
see all
Anti-XIAP (phospho S87) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - XIAP (phospho S87) antibody (ab59289)
Ab59289 at 1/50 dilution staining human skeletal muscle without (left) and with (right) immunizing peptide; paraffin embedded.
Western blot - XIAP (phospho S87) antibody (ab59289)
Anti-XIAP (phospho S87) antibody (ab59289) at 1 µg/ml + T24/83 (Human bladder carcinoma) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 55 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
References for Anti-XIAP (phospho S87) antibody (ab59289)
ab59289 has not yet been referenced specifically in any publications.
Publishing research using ab59289? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"