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Products:Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Overview
Product name
Anti-XK antibody
See all XK products (2) ...
Description
Rabbit polyclonal to XK
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chimpanzee
Immunogen
Synthetic peptide corresponding to a region within the N terminal amino acids 72-121 (LHLLQLGPLF RCFEVFCIYF QSGNNEEPYV SITKKRQMPK NGLSEEIEKE) of Human XK (NP_066569)
LHLLQLGPLF RCFEVFCIYF QSGNNEEPYV SITKKRQMPK NGLSEEIEKE
Positive control
MCF7 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
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Western blot - XK antibody (ab82686)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/12500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
XK is the Kell blood group 'precursor substance'. This protein has structural characteristics of prokaryotic and eukaryotic membrane transport proteins. The Kell blood group is constituted by two covalently linked antigens at the surface of red blood cells, Kell and KX. Whereas Kell is a metalloprotease with demonstrated in vitro enzymatic activity, the role of KX thereon, and/or alone, remains unknown, although its absence is linked to the McLeod syndrome, a neuroacanthocytosis. KX appears to be required for proper synthesis or presentation of the Kell antigens on the red blood cell surface.
Cellular localization
Membrane; Multi pass membrane protein
Alternative names
- Kell complex 37 kDa component antibody
- Membrane transport protein XK antibody
- XK related protein 1 antibody
see all
Database links
- Entrez Gene: 7504 Human
- Entrez Gene: 22439 Mouse
- Entrez Gene: 497078 Rat
- Omim: 314850 Human
- SwissProt: P51811 Human
- SwissProt: Q9QXY7 Mouse
- SwissProt: Q5GH61 Rat
- Unigene: 78919 Human
Anti-XK antibody images:
Western blot - XK antibody (ab82686)
Anti-XK antibody (ab82686) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 51 kDa
Observed band size : 51 kDa
Additional bands at : 25 kDa,31 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
References for Anti-XK antibody (ab82686)
ab82686 has not yet been referenced specifically in any publications.
Publishing research using ab82686? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"