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Anti-XPA antibody [12F5] (ab2352)

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Overview

Product name

Anti-XPA antibody [12F5]
See all XPA products (12) ...

Description

Mouse monoclonal [12F5] to XPA

Tested applications

ICC/IF, WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length protein (Human).

Positive control

Ls174T or MCF-7 cell lysate. Tonsil tissue section.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

12F5

Myeloma

unknown

Isotype

IgG2a

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution (PMID 19451271).
IHC-P: Use at a concentration of 2 - 4 µg/ml (30 minute at RT). Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

Tissue specificity

Expressed in various cell lines and in skin fibroblasts.

Involvement in disease

Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.

Sequence similarities

Belongs to the XPA family.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP23025 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA repair protein complementing XP A cells antibody
  • DNA repair protein complementing XP-A cells antibody
  • DNA repair protein complementing XPA cells antibody
  • Excision repair controlling antibody
  • Xeroderma pigmentosum 1 antibody
  • Xeroderma pigmentosum complementation group A antibody
  • Xeroderma pigmentosum group A complementing protein antibody
  • Xeroderma pigmentosum group A-complementing protein antibody
  • XP 1 antibody
  • XP1 antibody
  • xpa antibody
  • XPA_HUMAN antibody
  • Xpac antibody
see all

Database links

Anti-XPA antibody [12F5] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)

Ab2352 (1:50) staining human XPA in human tonsil tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References for Anti-XPA antibody [12F5] (ab2352)

This product has been referenced in:

  • Overmeer RMet al. Replication protein A safeguards genome integrity by controlling NER incision events. J Cell Biol 192:401-15 (2011). ICC/IF; Human.Read more (PubMed: 21282463) »
  • Gueranger Qet al. Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21398635) »

See all 4 publications for this product

Publishing research using ab2352? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"