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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)](/ps/datasheet/images/2/ab2352/XPA-Primary-antibodies-ab2352-1.jpg)
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Product Name
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XPA antibody [12F5]
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See all XPA antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [12F5] to XPA
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19451271).
IHC-P: Use at a concentration of 2 - 4 µg/ml (30 minute at RT). Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Ls174T or MCF-7 cell lysate.
Tonsil tissue section.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
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Relevance
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The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV- or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC 1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000 fold increased risk to develop skin cancer at sun-exposed areas.
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XPA antibody [12F5] (ab2352) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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12F5
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XPA antibody [12F5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XPA antibody [12F5] images, references, products related to ab2352 and other tools.
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XPA antibody [12F5] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPA antibody [12F5] (ab2352)
Ab2352 (1:50) staining human XPA in human tonsil tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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References for XPA antibody [12F5] (ab2352)
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This product has been used in:
Luijsterburg MS et al. Heterochromatin protein 1 is recruited to various types of DNA damage. J Cell Biol 185:577-86 (2009). ICC/IF. PubMed: 19451271
If you publish research using ab2352 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XPA
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XPA antibody [12F5] - more information
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Customer reviews (feedback) regarding XPA antibody [12F5] |
Customer FAQs regarding XPA antibody [12F5] |
Protocols for XPA antibody [12F5] |
Price and availability of products related to XPA antibody [12F5] |
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XPA antibody [12F5] - related products:
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XPA antibody [12F5] - other tools:
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Contact Abcam with a Technical Enquiry about XPA antibody [12F5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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