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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Overview
Product name
Anti-XPA antibody [2A4]
See all XPA products (12) ...
Description
Mouse monoclonal [2A4] to XPA
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (his-tagged)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Works on pure and partially purified protein, but not on crude cell extract. Can be used for nuclear staining of XPA in human cells.
Clonality
Monoclonal
Clone number
2A4
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Light chain type
unknown
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Target
Function
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
Tissue specificity
Expressed in various cell lines and in skin fibroblasts.
Involvement in disease
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
Sequence similarities
Belongs to the XPA family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23025
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
Database links
- Entrez Gene: 7507 Human
- Omim: 278700 Human
- SwissProt: P23025 Human
- Unigene: 654364 Human
References for Anti-XPA antibody [2A4] (ab20252)
ab20252 has not yet been referenced specifically in any publications.
Publishing research using ab20252? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"