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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Overview
Product name
Anti-XPA antibody [5A2]
See all XPA products (12) ...
Description
Mouse monoclonal [5A2] to XPA
Conjugation notes
-
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
His-tagged recombinant human XPA (Xeroderma Pigmentosum group A) protein
General notes
Works on pure and partially purified protein but not on crude cell extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5A2
Myeloma
Sp2
Isotype
IgG1
Light chain type
unknown
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Target
Function
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
Tissue specificity
Expressed in various cell lines and in skin fibroblasts.
Involvement in disease
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
Sequence similarities
Belongs to the XPA family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23025
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
Database links
- Entrez Gene: 7507 Human
- Omim: 278700 Human
- SwissProt: P23025 Human
- Unigene: 654364 Human
References for Anti-XPA antibody [5A2] (ab44)
This product has been referenced in:
- Wei Qet al. Expression of nucleotide excision repair proteins in lymphocytes as a marker of susceptibility to squamous cell carcinomas of the head and neck. Cancer Epidemiol Biomarkers Prev 14:1961-6 (2005).Read more (PubMed: 16103444) »
See 1 publication for this product
Publishing research using ab44? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"