|
|
|
|
Western blot - XPA antibody [5F12] (ab65963)
![Western blot - XPA antibody [5F12] (ab65963)](/ps/datasheet/images/ab65963_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
XPA antibody [5F12]
|
| |
See all XPA antibodies (11)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [5F12] to XPA
|
|
Immunogen
|
Recombinant full length XPA protein (Human)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms
(Due to sequence homology)
|
|
Epitope
|
Amino acids 30-47.
|
|
Tested applications
(see key)
|
ELISA, Inhib, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 - 1.0 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
Inhib: Inhibition of in vitro excision repair reaction and Inhibition of XPA interaction with ERCC1 and TFIIH. Use at an assay dependent dilutions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Crude extract of HeLa cells.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
|
|
Relevance
|
The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV- or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC 1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000 fold increased risk to develop skin cancer at sun-exposed areas.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
5F12
|
|
Isotype
|
IgG2b
|
|
Purity
|
Ion Exchange Chromatography
|
|
Storage buffer
|
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XPA antibody [5F12] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for XPA antibody [5F12] images, references, products related to ab65963 and other tools.
|
| |
XPA antibody [5F12] images:
|
|
|
| |
References for XPA antibody [5F12] (ab65963)
|
|
This product has been used in: (two most recent references)
Herrera M et al. Differences in repair of DNA cross-links between lymphocytes and epithelial tumor cells from colon cancer patients measured in vitro with the comet assay. Clin Cancer Res 15:5466-72 (2009). WB; Human. PubMed: 19690199
This clone has been used in:
If you publish research using ab65963 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to XPA
|
| |
XPA antibody [5F12] - more information
|
Customer reviews (feedback) regarding XPA antibody [5F12] |
Customer FAQs regarding XPA antibody [5F12] |
Protocols for XPA antibody [5F12] |
Price and availability of products related to XPA antibody [5F12] |
| |
XPA antibody [5F12] - related products:
|
|
|
| |
XPA antibody [5F12] - other tools:
|
Contact Abcam with a Technical Enquiry about XPA antibody [5F12] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - XPA antibody [5F12] (ab65963)](/index.html?datasheet=65963#ab65963_1.jpg)
Western blot - XPA antibody [5F12] (ab65963)