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Anti-XPA antibody [5F12] (ab65963)

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Overview

Product name

Anti-XPA antibody [5F12]
See all XPA products (12) ...

Description

Mouse monoclonal [5F12] to XPA

Tested applications

ELISA, WB, Inhibition Assaymore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Recombinant full length XPA protein (Human)

Epitope

Amino acids 30-47.

Positive control

Crude extract of HeLa cells.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Ion Exchange Chromatography

Clonality

Monoclonal

Clone number

5F12

Isotype

IgG2b

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair

  • Western blot - XPA antibody [5F12] (ab65963)Western blot - XPA antibody [5F12] (ab65963) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.1 - 1.0 µg/ml.Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).

  • ShowHide

    Inhibition Assay

     Inhib: Use at an assay d...Read more →

    Inhib: Use at an assay dependent dilution. (Inhibition of in vitro excision repair reaction and inhibition of XPA interaction with ERCC1 and TFIIH.)

Target

Function

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

Tissue specificity

Expressed in various cell lines and in skin fibroblasts.

Involvement in disease

Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.

Sequence similarities

Belongs to the XPA family.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP23025 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA repair protein complementing XP A cells antibody
  • DNA repair protein complementing XP-A cells antibody
  • DNA repair protein complementing XPA cells antibody
  • Excision repair controlling antibody
  • Xeroderma pigmentosum 1 antibody
  • Xeroderma pigmentosum complementation group A antibody
  • Xeroderma pigmentosum group A complementing protein antibody
  • Xeroderma pigmentosum group A-complementing protein antibody
  • XP 1 antibody
  • XP1 antibody
  • xpa antibody
  • XPA_HUMAN antibody
  • Xpac antibody
see all

Database links

Anti-XPA antibody [5F12] images:

  Western blot - XPA antibody [5F12] (ab65963)

Western blot - XPA antibody [5F12] (ab65963)

Anti-XPA antibody [5F12] (ab65963) at 0.1 µg/ml + Crude extract of HeLa cells at 200 µg

Predicted band size : 31 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-XPA antibody [5F12] (ab65963)

This product has been referenced in:

  • Herrera Met al. Differences in repair of DNA cross-links between lymphocytes and epithelial tumor cells from colon cancer patients measured in vitro with the comet assay. Clin Cancer Res 15:5466-72 (2009). WB; Human.Read more (PubMed: 19690199) »
  • Telen MJet al. A blood group-related polymorphism of CD44 abolishes a hyaluronan-binding consensus sequence without preventing hyaluronan binding. J Biol Chem 271:7147-53 (1996).Read more (PubMed: 8636151) »

See all 2 publications for this product

Publishing research using ab65963? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"