Overview

  • Product nameAnti-XPB antibodySee all XPB primary antibodies ...
  • Description
    Rabbit polyclonal to XPB
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region between residue 575 and 625 of Human XPB (NP_000113.1).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Pancreatic Islet Cell Tumor and Prostate Carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab85408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
    • Involvement in diseaseDefects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
      Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
    • Sequence similaritiesBelongs to the helicase family. RAD25/XPB subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localizationNucleus.
    • Target information above from: UniProt accession P19447 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Basic transcription factor 2 89 kDa subunit antibody
      • BTF 2 antibody
      • BTF2 antibody
      • BTF2 p89 antibody
      • DNA excision repair protein ERCC-3 antibody
      • DNA repair protein complementing XP-B cells antibody
      • ERCC 3 antibody
      • ERCC3 antibody
      • ERCC3_HUMAN antibody
      • Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibody
      • GTF2H antibody
      • RAD 25 antibody
      • RAD25 antibody
      • TFIIH 89 kDa subunit antibody
      • TFIIH antibody
      • TFIIH basal transcription factor complex 89 kDa subunit antibody
      • TFIIH basal transcription factor complex helicase XPB subunit antibody
      • TFIIH p89 antibody
      • Xeroderma pigmentosum group B-complementing protein antibody
      see all

    Anti-XPB antibody images

    • ab85408, at 1/250 dilution, staining XPB in formalin-fixed, paraffin-embedded human bladder cell carcinoma by Immunohistochemistry using DAB staining.
    • ab85408, at 1/500 dilution, staining XPB in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
    • Immunohistochemical analysis of Human hair follicle bulb tissue, staining XPB with ab85408.

      Antigen retrieval was performed by heat mediation in antigen target retrieval solution. Samples were blocked with goat serum followed by incubation with primary antibody overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.

    References for Anti-XPB antibody (ab85408)

    This product has been referenced in:
    • Yu M  et al. Deficiency in Nucleotide Excision Repair Family Gene Activity, Especially ERCC3, Is Associated with Non-Pigmented Hair Fiber Growth. PLoS One 7:e34185 (2012). IHC-P ; Human . Read more (PubMed: 22615732) »

    See 1 Publication for this product

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