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Overview

  • Product nameAnti-XPB antibodySee all XPB primary antibodies ...
  • Description
    Rabbit polyclonal to XPB
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to amino acids 521-712 of Human XPB (NP_000113).

  • Positive control293T, A431 and H1299 whole cell lysates; HeLa cells; HepG2, Molt-4 and Raji cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab97668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Predicted molecular weight: 89 kDa.
ICC/IF ICC/IF: 1/200.

Target

  • FunctionATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
  • Involvement in diseaseDefects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
    Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
  • Sequence similaritiesBelongs to the helicase family. RAD25/XPB subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P19447 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Basic transcription factor 2 89 kDa subunit antibodyBTF 2 antibodyBTF2 antibody
      BTF2 p89 antibodyDNA excision repair protein ERCC-3 antibodyDNA repair protein complementing XP-B cells antibodyERCC 3 antibodyERCC3 antibodyERCC3_HUMAN antibodyExcision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibodyGTF2H antibodyRAD 25 antibodyRAD25 antibodyTFIIH 89 kDa subunit antibodyTFIIH antibodyTFIIH basal transcription factor complex 89 kDa subunit antibodyTFIIH basal transcription factor complex helicase XPB subunit antibodyTFIIH p89 antibodyXeroderma pigmentosum group B-complementing protein antibody
    see all

Anti-XPB antibody images

  • Western blot - XPB antibody (ab97668)
    Western blot - XPB antibody (ab97668)
    All lanes : Anti-XPB antibody (ab97668) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 89 kDa
  • Immunocytochemistry/ Immunofluorescence - XPB antibody (ab97668)
    Immunocytochemistry/ Immunofluorescence - XPB antibody (ab97668)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97668 at 1/200 dilution. Lower image is merged with DNA probe.

References for Anti-XPB antibody (ab97668)

ab97668 has not yet been referenced specifically in any publications.

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