Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
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ab6264 has been referenced in 10 publications.
Publishing research using ab6264? Please let us know so that we can cite the reference in this datasheet
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Lane 1 : Anti-XPC antibody [3.26] (ab6264) at 1/1000 dilution
Lane 2 : Anti-XPC antibody [3.26] (ab6264) at 1/500 dilution
Lane 1 : Raji whole cell extract
Lane 2 : Raji whole cell extract
Predicted band size : 105 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Ab6264 staining human XPC in HeLa cells (left), and DAPI staining (right) by immunofluorescence.
ab6264 (4µg/ml) staining XPC in human tonsil, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Overlay histogram showing A431 cells stained with ab6264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6264, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Predicted band size : 105 kDa
Western blot image of Human XPC from HeLa whole cell lysate using:
Lane 1: Rabbit polyclonal ab21078
Lane 2: Rabbit polyclonal ab21082
Lane 3: Rabbit monoclonal ab6264
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