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Western blot - XPC antibody [3.26] (ab6264)
![Western blot - XPC antibody [3.26] (ab6264)](/ps/datasheet/images/ab6264_3.jpg)
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Immunocytochemistry/ Immunofluorescence - XPC antibody [3.26] (ab6264)
![Immunocytochemistry/ Immunofluorescence - XPC antibody [3.26] (ab6264)](/ps/datasheet/images/ab6264_4.jpg)
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Product Name
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XPC antibody [3.26]
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See all XPC antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3.26] to XPC
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Immunogen
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Recombinant full length human XPC protein expressed in E. coli.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: 1/100. IHC-P: 1/100. Perform antigen retrieval before commencing with IHC staining protocol. WB: 1/500 - 1/1000. Detects a band of approximately 113-120 kDa (predicted molecular weight: 105 kDa). Block with 1% BSA instead of milk.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji whole cell lysate, HeLa cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
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Relevance
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Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair. XPC may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage processing enzymes. Defects in XPC are a cause of xeroderma pigmentosum complementation group C (XPC); also known as xeroderma pigmentosum III (XP3). XPC is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3.26
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Myeloma
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NS1
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None Constituents: PBS
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Purification notes
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Purified from tissue culture supernatant.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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One customer reported cross-reaction of the antibody with p53.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XPC antibody [3.26] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XPC antibody [3.26] images, references, products related to ab6264 and other tools.
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XPC antibody [3.26] images:
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Western blot - XPC antibody [3.26] (ab6264)
Lane 1 : XPC antibody [3.26] (ab6264) at 1/1000 dilution Lane 2 : XPC antibody [3.26] (ab6264) at 1/500 dilution
Lane 1 : Raji whole cell extract Lane 2 : Raji whole cell extract
Predicted band size : 105 kDa Observed band size : 120 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - XPC antibody [3.26] (ab6264)
Ab6264 staining human XPC in HeLa cells (left), and DAPI staining (right) by immunofluorescence.
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References for XPC antibody [3.26] (ab6264)
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This product has been used in: (two most recent references)
Khan SG et al. XPC initiation codon mutation in xeroderma pigmentosum patients with and without neurological symptoms. DNA Repair (Amst) 8:114-25 (2009). PubMed: 18955168
Svendsen S et al. Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. BMC Cell Biol 9:17 (2008). ICC/IF; Human. PubMed: 18384686
See all 8 publication references for this product.
If you publish research using ab6264 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XPC
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XPC antibody [3.26] - more information
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Customer reviews (feedback) regarding XPC antibody [3.26] |
Customer FAQs regarding XPC antibody [3.26] |
Protocols for XPC antibody [3.26] |
Price and availability of products related to XPC antibody [3.26] |
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XPC antibody [3.26] - related products:
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XPC antibody [3.26] - other tools:
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Contact Abcam with a Technical Enquiry about XPC antibody [3.26] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"