XPC antibody [3.26] (ab6264)

Product Name 

XPC antibody [3.26]

Product type 

Primary antibodies

Description 

Mouse monoclonal [3.26] to XPC

Immunogen 

Recombinant full length human XPC protein expressed in E. coli.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100.
IHC-P: 1/100. Perform antigen retrieval before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 113-120 kDa (predicted molecular weight: 105 kDa). Block with 1% BSA instead of milk.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Raji whole cell lysate, HeLa cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair

Relevance 

Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair. XPC may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage processing enzymes. Defects in XPC are a cause of xeroderma pigmentosum complementation group C (XPC); also known as xeroderma pigmentosum III (XP3). XPC is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3.26

Myeloma 

NS1

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS

Purification notes 

Purified from tissue culture supernatant.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

One customer reported cross-reaction of the antibody with p53.

See below for XPC antibody [3.26] images, references, products related to ab6264 and other tools.

XPC antibody [3.26] images:

References for XPC antibody [3.26] (ab6264)

  This product has been used in: (two most recent references)

Khan SG et al. XPC initiation codon mutation in xeroderma pigmentosum patients with and without neurological symptoms. DNA Repair (Amst) 8:114-25 (2009). PubMed: 18955168

Svendsen S et al. Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. BMC Cell Biol 9:17 (2008). ICC/IF; Human. PubMed: 18384686

See all 8 publication references for this product.


If you publish research using ab6264 please let us know so that we can cite the reference on this datasheet.

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