Anti-XPF antibody [SPM228] (ab17798)

Overview

• Product nameAnti-XPF antibody [SPM228]See all XPF primary antibodies ...
• Description
  Mouse monoclonal [SPM228] to XPF
• Tested applicationsWB, IHC-P, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant human XPF protein

• Positive controlMCF-7 cells or human tonsil

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityPreadsorbed IgG fraction
• Clonality Monoclonal
• Clone numberSPM228
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Nucl. Excision Repair

Applications

Target

• FunctionStructure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
• Involvement in diseaseDefects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
  Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
• Sequence similaritiesBelongs to the XPF family.
  Contains 1 ERCC4 domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q92889 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2072 Human
  • Omim: 278760 Human
  • SwissProt: Q92889 Human
  • Unigene: 567265 Human

• Alternative names
  DNA excision repair protein ERCC 4 antibodyDNA excision repair protein ERCC-4 antibodyDNA excision repair protein ERCC4 antibody

  DNA repair endonuclease XPF antibodyDNA repair protein complementing XP F cells antibodyDNA repair protein complementing XP-F cells antibodyERCC 11 antibodyERCC 4 antibodyERCC11 antibodyERCC4 antibodyExcision repair complementing defective in Chinese hamster antibodyExcision repair cross complementing rodent repair deficiency complementation group 4 antibodyRAD 1 antibodyRAD1 antibodyXeroderma Pigmentosum Complementation group F antibodyXeroderma Pigmentosum Complementation group F antibodyXeroderma pigmentosum group F complementing protein antibodyXeroderma pigmentosum group F-complementing protein antibodyXPF_HUMAN antibody

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Anti-XPF antibody [SPM228] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPF antibody [SPM228] (ab17798)
  ab17798 XPF [SPM228] staining in formalin-fixed human tonsil tissue (1/50; 30min incubation at RT).
• 

  Western blot - XPF antibody [SPM228] (ab17798)
  Anti-XPF antibody [SPM228] (ab17798) at 1/25 dilution + MCF-7 cell lysate at 1 µg
  
  Predicted band size : 104 kDa
  Observed band size : 104 kDa
  
• 

  Flow Cytometry - Anti-XPF antibody [SPM228] (ab17798)
  Overlay histogram showing HeLa cells stained with ab17798 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17798, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.