Overview

  • Product nameAnti-XPF antibody [SPM228]See all XPF primary antibodies ...
  • Description
    Mouse monoclonal [SPM228] to XPF
  • Tested applicationsWB, IHC-P, IP, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human XPF protein

  • Positive control
    • MCF-7 cells or human tonsil

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityPreadsorbed IgG fraction
  • Clonality Monoclonal
  • Clone numberSPM228
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab17798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionStructure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
  • Involvement in diseaseDefects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
  • Sequence similaritiesBelongs to the XPF family.
    Contains 1 ERCC4 domain.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q92889 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DNA excision repair protein ERCC 4 antibody
    • DNA excision repair protein ERCC-4 antibody
    • DNA excision repair protein ERCC4 antibody
    • DNA repair endonuclease XPF antibody
    • DNA repair protein complementing XP F cells antibody
    • DNA repair protein complementing XP-F cells antibody
    • ERCC 11 antibody
    • ERCC 4 antibody
    • ERCC11 antibody
    • ERCC4 antibody
    • Excision repair complementing defective in Chinese hamster antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
    • excision repair cross-complementation group 4 antibody
    • FANCQ antibody
    • RAD 1 antibody
    • RAD1 antibody
    • Xeroderma Pigmentosum Complementation group F antibody
    • Xeroderma Pigmentosum Complementation group F antibody
    • Xeroderma pigmentosum group F complementing protein antibody
    • Xeroderma pigmentosum group F-complementing protein antibody
    • Xeroderma pigmentosum VI antibody
    • XP, group G antibody
    • XP6 antibody
    • XPF_HUMAN antibody
    see all

Anti-XPF antibody [SPM228] images

  • ab17798 staining XPF (green) in Human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Blue - nuclei (DAPI).

    See Abreview

  • ab17798 XPF [SPM228] staining in formalin-fixed human tonsil tissue (1/50; 30min incubation at RT).
  • Anti-XPF antibody [SPM228] (ab17798) at 1/25 dilution + MCF-7 cell lysate at 1 µg

    Predicted band size : 104 kDa
    Observed band size : 104 kDa
  • Overlay histogram showing HeLa cells stained with ab17798 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17798, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-XPF antibody [SPM228] (ab17798)

This product has been referenced in:
  • Duquette ML  et al. CtIP is required to initiate replication-dependent interstrand crosslink repair. PLoS Genet 8:e1003050 (2012). WB ; Human . Read more (PubMed: 23144634) »
  • Vaezi A  et al. XPF expression correlates with clinical outcome in squamous cell carcinoma of the head and neck. Clin Cancer Res 17:5513-22 (2011). Read more (PubMed: 21737503) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Submitted Dec 13 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry regarding ab17798. I am very sorry to hear about this problem.

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Application Western blot
Sample Human Cell lysate - whole cell (A549 cells)
Loading amount 20 µg
Specification A549 cells
Treatment 10 nM siRNA to XPF
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Apr 17 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted Dec 21 2011

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Nov 01 2011

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
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Submitted Apr 06 2011

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Dr. Svetlana Khoronenkova

Verified customer

Submitted Sep 13 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Lung Carcinoma)
Specification Human Lung Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Casein as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
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Verified customer

Submitted Oct 27 2009

Thank you for your enquiry. The antibody is IgG2a kappa. I have updated the datasheet for future reference. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"