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ab1838 has been referenced in 13 publications.
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ab1838 - immunohistochemistry
Formalin fixed paraffin embedded human testis stained with XRCC1, using ABC and AEC chromogen.
All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution
Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA
Secondary
HRP conjugated rabbit ant-mouse antibody at 1/2500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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