Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Thanks with your help with this. I will take up the offer and use test it on mouse. |
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ANSWER: |
Thank you for your reply. |
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Is ab1838 tested in mouse? |
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ANSWER: |
Thank you for your telephone call earlier today. |
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ANSWER: |
Thank you for your enquiry. The concentration of the latest batch of Mouse monoclonal [33-2-5] to XRCC1 (ab1838) is 100ugml-1. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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What is the concentration? Abcam lot 136702. |
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ANSWER: |
The concentration is 100 ug/ml. |
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I just bought this 33-2-5 mouse monoclonal to XRCc1 antibody (ab1838).I was just wondering what is the concentration of this antibody (I just got the amount-250ul,but not the concentration (mg/ml).Please let me know so that i can detect my working solution for western blots.Thanks
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ANSWER: |
Thank you for your enquiry. The concentration of ab1838, anti-XRCc1 is 100 ug/ml. Should you require further assistance please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab1838 - immunohistochemistry
Formalin fixed paraffin embedded human testis stained with XRCC1, using ABC and AEC chromogen.
All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution
Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA
Secondary
HRP conjugated rabbit ant-mouse antibody at 1/2500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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