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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-XRCC1 antibody
See all XRCC1 products (9) ...
Description
Rabbit polyclonal to XRCC1
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human XRCC1.
(Peptide available as ab49616.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; HEK293; HepG2; MCF7; SHSY-5Y; and U2OS.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: 1/250. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similarities
Contains 2 BRCT domains.
Post-translational
modifications
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.
Cellular localization
Nucleus. Accumulates at sites of DNA damage.
Target information above from: UniProt accessionP18887
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
Database links
- Entrez Gene: 616905 Cow
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q6IBS4 Human
- SwissProt: Q60596 Mouse
see all
Anti-XRCC1 antibody images:
Western blot - XRCC1 antibody (ab47920)
All lanes : Anti-XRCC1 antibody (ab47920) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
The band seen at 80 kDa is consistent with the banding pattern observed for other commercially available antibodies to XRCC1.
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
ICC/IF image of ab47920 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-Tween20 0.1% (20 min) and incubated with the antibody (ab47920, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). Antibodies were diluted in 1% BSA, 1% normal goat serum in PBS-Tween.
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
ab47920 (1/200) staining XRCC1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody (ab47920)
ab47920 staining XRCC1 in human tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/50 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-XRCC1 antibody (ab47920)
This product has been referenced in:
- Saha Tet al. Transcriptional regulation of the base excision repair pathway by BRCA1. J Biol Chem 285:19092-105 (2010). WB; Human.Read more (PubMed: 20185827) »
See 1 publication for this product
Publishing research using ab47920? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"