|
|
|
|
Western blot - XRCC1 antibody (ab47920)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
(enlarge)
|
|
|
|
|
Product Name
|
XRCC1 antibody
|
| |
See all XRCC1 antibodies (11)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to XRCC1
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human XRCC1. (Peptide available as ab49616.)
|
|
Reacts with
|
Hu, Ms
|
|
Predicted to react
(species key)
|
Rat
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: 1/250. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in the following whole cell lysates:
HeLa; Jurkat; A431; HEK293; HepG2; MCF7; SHSY-5Y; and U2OS.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
|
|
Relevance
|
XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for XRCC1 antibody images, references, products related to ab47920 and other tools.
|
| |
XRCC1 antibody images:
|
 Western blot - XRCC1 antibody (ab47920)
All lanes : XRCC1 antibody (ab47920) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
The band seen at 80 kDa is consistent with the banding pattern observed for other commercially available antibodies to XRCC1.
|
| |
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
ICC/IF image of ab47920 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-Tween20 0.1% (20 min) and incubated with the antibody (ab47920, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). Antibodies were diluted in 1% BSA, 1% normal goat serum in PBS-Tween.
|
| |
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab47920)
ab47920 (1/200) staining XRCC1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRCC1 antibody (ab47920)
ab47920 staining XRCC1 in human tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/50 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
|
| |
| |
|
Search PubMed (MEDLINE) for references to XRCC1
|
| |
XRCC1 antibody - more information
|
Customer reviews (feedback) regarding XRCC1 antibody |
Customer FAQs regarding XRCC1 antibody |
Protocols for XRCC1 antibody |
Price and availability of products related to XRCC1 antibody |
| |
XRCC1 antibody - related products:
|
|
|
| |
XRCC1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about XRCC1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
