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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-XRCC1 antibody
See all XRCC1 products (9) ...
Description
Rabbit polyclonal to XRCC1
Specificity
ab58465 detects endogenous levels of total XRCC1 protein.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from internal sequence of human XRCC1.
Positive control
WB: Extracts from Jurkat cells. IHC-P: human lung carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similarities
Contains 2 BRCT domains.
Post-translational
modifications
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.
Cellular localization
Nucleus. Accumulates at sites of DNA damage.
Target information above from: UniProt accessionP18887
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
Database links
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q6IBS4 Human
- SwissProt: Q60596 Mouse
- SwissProt: Q9CQD7 Mouse
see all
Anti-XRCC1 antibody images:
Western blot - XRCC1 antibody (ab58465)
All lanes : Anti-XRCC1 antibody (ab58465) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunizing peptide
Predicted band size : 70 kDa
Observed band size : 70 kDa
Immunohistochemistry (Paraffin-embedded sections) - XRCC1 antibody (ab58465)
Ab58465, at 1/50 dilution, staining paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
References for Anti-XRCC1 antibody (ab58465)
ab58465 has not yet been referenced specifically in any publications.
Publishing research using ab58465? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"