|
|
|
|
Western blot - XRCC1 antibody (ab58465)
(enlarge)
|
|
Immunohistochemistry (Paraffin-embedded sections) - XRCC1 antibody (ab58465)
(enlarge)
|
|
|
|
|
Product Name
|
XRCC1 antibody
|
| |
See all XRCC1 antibodies (11)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to XRCC1
|
|
Immunogen
|
Synthetic peptide derived from internal sequence of human XRCC1.
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
ab58465 detects endogenous levels of total XRCC1 protein.
|
|
Tested applications
(see key)
|
ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
WB: Extracts from Jurkat cells.
IHC-P: human lung carcinoma tissue.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
|
|
Relevance
|
XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for XRCC1 antibody images, references, products related to ab58465 and other tools.
|
| |
XRCC1 antibody images:
|
 Western blot - XRCC1 antibody (ab58465)
All lanes : XRCC1 antibody (ab58465) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunizing peptide
Predicted band size : 70 kDa
Observed band size : 70 kDa
|
| |
Immunohistochemistry (Paraffin-embedded sections) - XRCC1 antibody (ab58465)
Ab58465, at 1/50 dilution, staining paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
|
| |
| |
|
Search PubMed (MEDLINE) for references to XRCC1
|
| |
XRCC1 antibody - more information
|
Customer reviews (feedback) regarding XRCC1 antibody |
Customer FAQs regarding XRCC1 antibody |
Protocols for XRCC1 antibody |
Price and availability of products related to XRCC1 antibody |
| |
XRCC1 antibody - related products:
|
|
|
| |
XRCC1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about XRCC1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
