Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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ab9147 has been referenced in 7 publications.
Publishing research using ab9147? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size : 70 kDa
Observed band size : 76,90 kDa (why is the actual band size different from the predicted?)
Detection: ECL.
ab9147 staining human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
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