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Western blot - XRCC1 antibody (ab9147)
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Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab9147)
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Product Name
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XRCC1 antibody
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See all XRCC1 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XRCC1
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Immunogen
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Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Hm
(Due to sequence homology)
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Specificity
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This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/200 - 1/500.
IP: Use at a concentration of 2 - 6 µg/ml.
WB: 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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Notes
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Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRCC1 antibody images, references, products related to ab9147 and other tools.
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XRCC1 antibody images:
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 Western blot - XRCC1 antibody (ab9147)
All lanes : XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size : 70 kDa
Observed band size : 76,90 kDa (why is the actual band size different from the predicted?)
Detection: ECL.
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Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab9147)
ab9147 staining human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary. This image is courtesy of an Abreview submitted by Dr Alexander Rapp
See Abreview
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References for XRCC1 antibody (ab9147)
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This product has been used in: (two most recent references)
Sugino Y et al. Septins as diagnostic markers for a subset of human asthenozoospermia. J Urol 180:2706-9 (2008). PubMed: 18951558
Bekker-Jensen S et al. Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks. J Biol Chem 282:19638-43 (2007). PubMed: 17507382
See all 4 publication references for this product.
If you publish research using ab9147 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XRCC1
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XRCC1 antibody - more information
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Customer reviews (feedback) regarding XRCC1 antibody |
Customer FAQs regarding XRCC1 antibody |
Protocols for XRCC1 antibody |
Price and availability of products related to XRCC1 antibody |
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XRCC1 antibody - related products:
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XRCC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRCC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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