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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-XRCC1 antibody
See all XRCC1 products (9) ...
Description
Rabbit polyclonal to XRCC1
Specificity
This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein.
Tested applications
IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Hamster
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
General notes
Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 2 - 6 µg/ml.
WB: 1/1000 - 1/10000.Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
ICC/IF: 1/200 - 1/500.
Target
Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similarities
Contains 2 BRCT domains.
Post-translational
modifications
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.
Cellular localization
Nucleus. Accumulates at sites of DNA damage.
Target information above from: UniProt accessionP18887
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- RCC antibody
see all
Database links
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q6IBS4 Human
- SwissProt: Q60596 Mouse
- SwissProt: Q9CQD7 Mouse
see all
Anti-XRCC1 antibody images:
Western blot - XRCC1 antibody (ab9147)
All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size : 70 kDa
Observed band size : 76,90 kDa (why is the actual band size different from the predicted?)
Detection: ECL.
Immunocytochemistry/ Immunofluorescence - XRCC1 antibody (ab9147)
ab9147 staining human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
References for Anti-XRCC1 antibody (ab9147)
This product has been referenced in:
- Slupianek Aet al. BCR/ABL stimulates WRN to promote survival and genomic instability. Cancer Res 71:842-51 (2011). WB; Mouse.Read more (PubMed: 21123451) »
- Saribasak Het al. XRCC1 suppresses somatic hypermutation and promotes alternative nonhomologous end joining in Igh genes. J Exp Med 208:2209-16 (2011). IP.Read more (PubMed: 21967769) »
See all 7 publications for this product
Publishing research using ab9147? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"