|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254)
](\ps\datasheet\images\20\ab20254\XRCC3-Primary-antibodies-ab20254-1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
XRCC3 antibody [XRCC3 10F1/6]
|
| |
See all XRCC3 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [XRCC3 10F1/6] to XRCC3
|
|
Immunogen
|
Full length protein (human) which has been his-tagged, overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
|
|
Relevance
|
The XRCC3 gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. It is essential for repairing the double strand DNA breaks by homologous recombination between sister chromatids. It is homologous to RAD51. Five human homologues of RAD51; XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complex or several smaller complexes.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
XRCC3 10F1/6
|
|
Myeloma
|
Sp2/0-Ag14
|
|
Isotype
|
IgG1
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: None
Constituents: PBS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC3 antibody [XRCC3 10F1/6] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for XRCC3 antibody [XRCC3 10F1/6] images, references, products related to ab20254 and other tools.
|
| |
XRCC3 antibody [XRCC3 10F1/6] images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254)
Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
| |
|
Search PubMed (MEDLINE) for references to XRCC3
|
| |
XRCC3 antibody [XRCC3 10F1/6] - more information
|
Customer reviews (feedback) regarding XRCC3 antibody [XRCC3 10F1/6] |
Customer FAQs regarding XRCC3 antibody [XRCC3 10F1/6] |
Protocols for XRCC3 antibody [XRCC3 10F1/6] |
Price and availability of products related to XRCC3 antibody [XRCC3 10F1/6] |
| |
XRCC3 antibody [XRCC3 10F1/6] - related products:
|
|
|
| |
XRCC3 antibody [XRCC3 10F1/6] - other tools:
|
Contact Abcam with a Technical Enquiry about XRCC3 antibody [XRCC3 10F1/6] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
](/index.html?datasheet=20254#XRCC3-Primary-antibodies-ab20254-1.jpg)
