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Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)

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Overview

Product name

Anti-XRCC3 antibody [XRCC3 10F1/6]
See all XRCC3 products (4) ...

Description

Mouse monoclonal [XRCC3 10F1/6] to XRCC3

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length protein (human) which has been his-tagged, overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.

Positive control

Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

XRCC3 10F1/6

Myeloma

Sp2/0-Ag14

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254) image (enlarge)

  • Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

Sequence similarities

Belongs to the RecA family. RAD51 subfamily.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.

Target information above from: UniProt accessionO43542 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA repair protein XRCC3 antibody
  • RAD51 like antibody
  • X ray repair complementing defective repair in Chinese hamster antibody
  • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
  • X ray repair cross complementing protein 3 antibody
  • X-ray repair cross-complementing protein 3 antibody
  • XRCC 3 antibody
  • XRCC3 antibody
  • XRCC3_HUMAN antibody
see all

Database links

Anti-XRCC3 antibody [XRCC3 10F1/6] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-XRCC3 antibody [XRCC3 10F1/6](ab20254)

Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)

Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)



Predicted band size : 38 kDa

See Abreview

References for Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)

ab20254 has not yet been referenced specifically in any publications.

Publishing research using ab20254? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"