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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-XRCC3 antibody
See all XRCC3 products (4) ...
Description
Rabbit polyclonal to XRCC3
Specificity
This antibody reacts with a 38 kD protein, known as XRCC3 (X-Ray Cross Complementing 3).
Tested applications
WB, IHC-P, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human).
Positive control
Testis.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-P
IHC-Fr
IP
Application notes
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
WB: 1/100 - 1/250. Predicted molecular weight: 38 kDa.
IP: 1/100 - 1/250.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
Target information above from: UniProt accessionO43542
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein XRCC3 antibody
- RAD51 like antibody
- X ray repair complementing defective repair in Chinese hamster antibody
see all
Database links
- Entrez Gene: 7517 Human
- Omim: 600675 Human
- SwissProt: O43542 Human
- Unigene: 592325 Human
References for Anti-XRCC3 antibody (ab2368)
ab2368 has not yet been referenced specifically in any publications.
Publishing research using ab2368? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"