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Product Name
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XRCC3 antibody
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See all XRCC3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XRCC3
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Immunogen
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Synthetic peptide, corresponding to amino acids 328-346 of Human XRCC3.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for XRCC3. No cross reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C or RAD51D.
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000. Predicted molecular weight: 38 kDa.
By Western blot, this antibody recognizes a band at 38kDa representing XRCC3.There are multiple bands for TK6 and HeLa cell extracts (this is normal and seen with XRCC3 recombinant protein). The band just above the XRCC3 band does notappear in nuclear extracts of TK6 cells (untested in HeLa), but theother two higher molecular weight bands are still present. We do notknow, however, what the higher molecular weight bands represent.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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The XRCC3 gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. It is essential for repairing the double strand DNA breaks by homologous recombination between sister chromatids. It is homologous to RAD51. Five human homologues of RAD51; XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complex or several smaller complexes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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5.300 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for XRCC3 antibody (ab6494)
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This product has been used in: (two most recent references)
Ning H et al. Identification of an aberrant cell line among human adipose tissue-derived stem cell isolates. Differentiation 77:172-80 (2009). PubMed: 19281777
Yoshioka K et al. ATR kinase activation mediated by MutSalpha and MutLalpha in response to cytotoxic O6-methylguanine adducts. Mol Cell 22:501-10 (2006). PubMed: 16713580
See all 5 publication references for this product.
If you publish research using ab6494 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XRCC3
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XRCC3 antibody - more information
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Customer reviews (feedback) regarding XRCC3 antibody |
Customer FAQs regarding XRCC3 antibody |
Protocols for XRCC3 antibody |
Price and availability of products related to XRCC3 antibody |
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XRCC3 antibody - related products:
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XRCC3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRCC3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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