|
|
|
|
Western blot - XRCC4 antibody (ab145)
(enlarge)
|
|
|
|
|
Product Name
|
XRCC4 antibody - ChIP Grade
|
| |
See all XRCC4 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to XRCC4 - ChIP Grade
|
|
Immunogen
|
Recombinant full length protein (Human).
|
|
Reacts with
(species key)
|
Hu, Rat
|
|
Specificity
|
This antibody recognises the XRCC4 protein (X ray cross complementation protein). XRCC4 is a ubiquitous protein reported to have a role in DNA double-strand break repair and in V(D)J recombination.
|
|
Tested applications
(see key)
|
ChIP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 17486112).
WB: 1/2000. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
|
|
Relevance
|
Human XRCC4 gene restores DNA double-strand break repair and the ability to support V(D)J recombination of transiently introduced substrates in the XR-1 CHO cell line.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
serum with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC4 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for XRCC4 antibody - ChIP Grade images, references, products related to ab145 and other tools.
|
| |
XRCC4 antibody - ChIP Grade images:
|
 Western blot - XRCC4 antibody (ab145)
XRCC4 antibody - ChIP Grade (ab145) at 1/2000 dilution + HeLa nuclear extract
Secondary
SHEEP ANTI RABBIT IgG:HRP
Predicted band size : 38 kDa
|
| |
References for XRCC4 antibody - ChIP Grade (ab145)
|
|
This product has been used in: (two most recent references)
Berkovich E et al. Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. Nat Protoc 3:915-22 (2008). PubMed: 18451799
Berkovich E et al. Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair. Nat Cell Biol 9:683-90 (2007). ChIP; Human. PubMed: 17486112
See all 5 publication references for this product.
If you publish research using ab145 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to XRCC4
|
| |
XRCC4 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding XRCC4 antibody - ChIP Grade |
Customer FAQs regarding XRCC4 antibody - ChIP Grade |
Protocols for XRCC4 antibody - ChIP Grade |
Price and availability of products related to XRCC4 antibody - ChIP Grade |
| |
XRCC4 antibody - ChIP Grade - related products:
|
|
|
| |
XRCC4 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about XRCC4 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
