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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
Overview
Product name
Anti-XRCC4 antibody - ChIP Grade
See all XRCC4 products (4) ...
Description
Rabbit polyclonal to XRCC4 - ChIP Grade
Specificity
This antibody recognises the XRCC4 protein (X ray cross complementation protein). XRCC4 is a ubiquitous protein reported to have a role in DNA double-strand break repair and in V(D)J recombination.
Tested applications
ChIP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.1% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Western blot - XRCC4 antibody (ab145)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 17486112)
WB: 1/2000Predicted molecular weight: 38 kDa.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the XRCC4 family.
Post-translational
modifications
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13426
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA double-strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
see all
Database links
- Entrez Gene: 7518 Human
- Entrez Gene: 309995 Rat
- Omim: 194363 Human
- SwissProt: Q13426 Human
- SwissProt: Q5XI44 Rat
- Unigene: 567359 Human
Anti-XRCC4 antibody - ChIP Grade images:
Western blot - XRCC4 antibody (ab145)
Anti-XRCC4 antibody - ChIP Grade (ab145) at 1/2000 dilution + HeLa nuclear extract
Secondary
SHEEP ANTI RABBIT IgG:HRP
Predicted band size : 38 kDa
References for Anti-XRCC4 antibody - ChIP Grade (ab145)
This product has been referenced in:
- Massip Let al. Deciphering the chromatin landscape induced around DNA double strand breaks. Cell Cycle 9:2963-72 (2010). ChIP; Human.Read more (PubMed: 20714222) »
- Berkovich Eet al. Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. Nat Protoc 3:915-22 (2008).Read more (PubMed: 18451799) »
See all 6 publications for this product
Publishing research using ab145? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"