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Western blot - XRCC4 antibody (ab2857)
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Immunocytochemistry/ Immunofluorescence - XRCC4 antibody (ab2857)
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Product Name
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XRCC4 antibody
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See all XRCC4 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to XRCC4
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Immunogen
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Recombinant protein encoding an amino terminalfragment of human XRCC4 purified from E. coli.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
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Relevance
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Human XRCC4 gene restores DNA double-strand break repair and the ability to support V(D)J recombination of transiently introduced substrates in the XR-1 CHO cell line.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRCC4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRCC4 antibody images, references, products related to ab2857 and other tools.
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XRCC4 antibody images:
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 Western blot - XRCC4 antibody (ab2857)
Predicted band size : 38 kDa
ab2857 at a 1:1000 dilution staining ~46 kDa XRCC 4 in HeLa whole cell lysate (30 µg/ml) by Western blot.
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Immunocytochemistry/ Immunofluorescence - XRCC4 antibody (ab2857)
Human HCT116 cells stained with ab2857. This picture was kindly submitted as part of the reveiw by Patrick Calsou.
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References for XRCC4 antibody (ab2857)
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This product has been used in: (two most recent references)
Herrmann G et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res 35:2321-32 (2007). PubMed: 17389648
Muylaert I & Elias P Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex virus type I DNA replication. J Biol Chem 282:10865-72 (2007). PubMed: 17296606
See all 3 publication references for this product.
If you publish research using ab2857 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to XRCC4
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XRCC4 antibody - more information
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Customer reviews (feedback) regarding XRCC4 antibody |
Customer FAQs regarding XRCC4 antibody |
Protocols for XRCC4 antibody |
Price and availability of products related to XRCC4 antibody |
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XRCC4 antibody - related products:
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XRCC4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRCC4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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