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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
Overview
Product name
Anti-XRCC4 antibody
See all XRCC4 products (4) ...
Description
Rabbit polyclonal to XRCC4
Specificity
ab58468 detects endogenous levels of total XRCC4 protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from internal sequence of human XRCC4
Positive control
WB: Extracts from COLO205 cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
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Western blot - XRCC4 antibody (ab58468)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the XRCC4 family.
Post-translational
modifications
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13426
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA double-strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
see all
Database links
- Entrez Gene: 7518 Human
- Omim: 194363 Human
- SwissProt: Q13426 Human
- Unigene: 567359 Human
Anti-XRCC4 antibody images:
Western blot - XRCC4 antibody (ab58468)
All lanes : Anti-XRCC4 antibody (ab58468) at 1/500 dilution
Lane 1 : extracts from COLO205 cells with no immunising peptide
Lane 2 : extracts from COLO205 cells with immunising peptide
Predicted band size : 38 kDa
References for Anti-XRCC4 antibody (ab58468)
ab58468 has not yet been referenced specifically in any publications.
Publishing research using ab58468? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"