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What is the chance that this antibody will cross react with rat protein? |
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ANSWER: |
The human epitope recognized by ab72181 is identical to the homologous rat epitope at 14 of 15 amino acids. In cases of even a one amino acid mismatch it can still be difficult to predict the chance for cross-species reactivity. But since it is a polyclonal antibody the chances are good ab72181 will cross react with rat protein.
We have a testing discount program you may be interested in. If you purchase a vial of ab72181 and test it in your rat samples and use our Abreview customer comment system to submit a short review of your experience, I can set up a discount code for you that will be good for a free antibody after we receive your Abreview.
Please contact me if you are interested in this promotion or would like further information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-XRN2 antibody (ab72181) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 170 kDa,300 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Detection of Human XRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72181 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
ICC/IF image of ab72181 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72181, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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