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Western blot - XRN2 antibody (ab72181)
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Immunoprecipitation - XRN2 antibody (ab72181)
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Product Name
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XRN2 antibody
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See all XRN2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XRN2
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Immunogen
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Synthetic peptide corresponding to a region between residues 900 and 950 of human XRN2 (NP_036387.2)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa and 293T whole cell lysates.
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Cellular localization
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Nucleus, nucleolus.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRN2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRN2 antibody images, references, products related to ab72181 and other tools.
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XRN2 antibody images:
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 Western blot - XRN2 antibody (ab72181)
All lanes : XRN2 antibody (ab72181) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 170 kDa,300 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - XRN2 antibody (ab72181)
Detection of Human XRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72181 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
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Search PubMed (MEDLINE) for references to XRN2
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XRN2 antibody - more information
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Customer reviews (feedback) regarding XRN2 antibody |
Customer FAQs regarding XRN2 antibody |
Protocols for XRN2 antibody |
Price and availability of products related to XRN2 antibody |
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XRN2 antibody - related products:
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XRN2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRN2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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