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All lanes : Anti-XRN2 antibody (ab72284) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 109 kDa
Observed band size : 109,115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
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