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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-XRN2 antibody
See all XRN2 products (5) ...
Description
Rabbit polyclonal to XRN2
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residues 25 and 75 of human XRN2 (NP_036387.2).
Positive control
Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - XRN2 antibody (ab72284)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000 - 1/10000. Detects bands of approximately 109 & 115 kDa (predicted molecular weight: 109 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Cellular localization
Nucleus, nucleolus.
Alternative names
- 5' 3' exoribonuclease 2 antibody
- DHM1 like protein antibody
- DHP protein antibody
see all
Database links
- Entrez Gene: 22803 Human
- Entrez Gene: 24128 Mouse
- Omim: 608851 Human
- SwissProt: Q9H0D6 Human
- SwissProt: Q9DBR1 Mouse
Anti-XRN2 antibody images:
Western blot - XRN2 antibody (ab72284)
All lanes : Anti-XRN2 antibody (ab72284) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 109 kDa
Observed band size : 109,115 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
References for Anti-XRN2 antibody (ab72284)
This product has been referenced in:
- Ternette Net al. Label-free quantitative proteomics reveals regulation of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and 5'-3'-exoribonuclease 2 (XRN2) during respiratory syncytial virus infection. Virol J 8:442 (2011). WB; Human.Read more (PubMed: 21933386) »
See 1 publication for this product
Publishing research using ab72284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"