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All lanes : Anti-XRN2 antibody (ab72307) at 0.04 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH 3T3 cells at 50 µg
Predicted band size : 109 kDa
Observed band size : 109,117 kDa (why is the actual band size different from the predicted?)
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