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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
(enlarge)
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Product Name
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XRN2 antibody
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See all XRN2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XRN2
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Immunogen
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Synthetic peptide corresponding to a region between residues 900 and 950 human XRN2 (NP_036387.2).
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100.
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
ab74408 is likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human tissues: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma.
Mouse tissues: Colon Carcinoma CT26, Teratoma.
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Cellular localization
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Nucleus, nucleolus.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab74408 was affinity purified using an epitope specific to XRN2 immobilized on solid support.
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Form
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Liquid
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRN2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRN2 antibody images, references, products related to ab74408 and other tools.
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XRN2 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded human ovarian carcinoma tissue by Immunohistochemistry. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded mouse teratoma tissue by Immunohistochemistry. Detection: DAB staining.
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Immunocytochemistry/ Immunofluorescence - XRN2 antibody (ab74408)
ab74408, at 1/100 dilution, staining XRN2 in human breast carcinoma tissue by Immunofluorescence. Detection: Red fluorescent Alexa Fluor 555 goat anti rabbit IgG at 1/500.
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Search PubMed (MEDLINE) for references to XRN2
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XRN2 antibody - more information
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Customer reviews (feedback) regarding XRN2 antibody |
Customer FAQs regarding XRN2 antibody |
Protocols for XRN2 antibody |
Price and availability of products related to XRN2 antibody |
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XRN2 antibody - related products:
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XRN2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRN2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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