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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-XRN2 antibody
See all XRN2 products (5) ...
Description
Rabbit polyclonal to XRN2
Tested applications
IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residues 900 and 950 human XRN2 (NP_036387.2).
Positive control
Human tissues: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma. Mouse tissues: Colon Carcinoma CT26, Teratoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab74408 was affinity purified using an epitope specific to XRN2 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/100.
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
ab74408 is likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Cellular localization
Nucleus, nucleolus.
Alternative names
- DHM1 like protein antibody
- DHP protein antibody
- RP23 236O6.1 antibody
- XRN 2 antibody
Database links
- Entrez Gene: 22803 Human
- Entrez Gene: 24128 Mouse
- Omim: 608851 Human
- SwissProt: Q9H0D6 Human
- SwissProt: Q9DBR1 Mouse
Anti-XRN2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded human ovarian carcinoma tissue by Immunohistochemistry. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74408)
ab74408, at 1/250 dilution, staining XRN2 in formalin fixed, paraffin embedded mouse teratoma tissue by Immunohistochemistry. Detection: DAB staining.
Immunocytochemistry/ Immunofluorescence - XRN2 antibody (ab74408)
ab74408, at 1/100 dilution, staining XRN2 in human breast carcinoma tissue by Immunofluorescence. Detection: Red fluorescent Alexa Fluor 555 goat anti rabbit IgG at 1/500.
References for Anti-XRN2 antibody (ab74408)
ab74408 has not yet been referenced specifically in any publications.
Publishing research using ab74408? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"