Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-XRN2 antibody
See all XRN2 products (5) ...
Description
Rabbit polyclonal to XRN2
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human, African Green Monkey
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from an internal sequence of human XRN2.
Positive control
Human breast carcinoma tissue and COS7 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Predicted molecular weight: 109 kDa.
IHC-P: 1/50 - 1/100.
Target
Relevance
XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Cellular localization
Nucleus, nucleolus.
Alternative names
- DHM1 like protein antibody
- DHP protein antibody
- RP23 236O6.1 antibody
- XRN 2 antibody
Database links
- Entrez Gene: 22803 Human
- Entrez Gene: 24128 Mouse
- Omim: 608851 Human
- SwissProt: Q9H0D6 Human
- SwissProt: Q9DBR1 Mouse
Anti-XRN2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74799)
ab74799 at 1/50 dilution staining XRN2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - XRN2 antibody (ab74799)
All lanes : Anti-XRN2 antibody (ab74799) at 1/500 dilution
Lane 1 : COS7 cell extracts
Lane 2 : COS7 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-XRN2 antibody (ab74799)
ab74799 has not yet been referenced specifically in any publications.
Publishing research using ab74799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"