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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74799)
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Western blot - XRN2 antibody (ab74799)
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Product Name
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XRN2 antibody
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See all XRN2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to XRN2
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Immunogen
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Synthetic peptide derived from an internal sequence of human XRN2.
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Reacts with
(species key)
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Hu, Ms, AGMk
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 109 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue and COS7 cell extracts.
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Cellular localization
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Nucleus, nucleolus.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XRN2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for XRN2 antibody images, references, products related to ab74799 and other tools.
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XRN2 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XRN2 antibody (ab74799)
ab74799 at 1/50 dilution staining XRN2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - XRN2 antibody (ab74799)
All lanes : XRN2 antibody (ab74799) at 1/500 dilution
Lane 1 : COS7 cell extracts
Lane 2 : COS7 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to XRN2
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XRN2 antibody - more information
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Customer reviews (feedback) regarding XRN2 antibody |
Customer FAQs regarding XRN2 antibody |
Protocols for XRN2 antibody |
Price and availability of products related to XRN2 antibody |
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XRN2 antibody - related products:
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XRN2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about XRN2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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