Overview

  • Product nameXanthine Oxidase Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 10 mU/ml
  • Range
    10 mU/ml - 1000 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Xanthine Oxidase Assay Kit is an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceXanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
  • Cellular localizationCytoplasm. Peroxisome. Secreted.
  • Alternative names
    • Xanthine dehydrogenase/oxidase
    • Xanthine oxidoreductase
    • XO
    • XOR

Applications

Our Abpromise guarantee covers the use of ab102522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Xanthine Oxidase Assay Kit images

  • Xanthine Oxidase Positive Control

Protocols

References for Xanthine Oxidase Assay Kit (ab102522)

This product has been referenced in:
  • Paneni F  et al. Deletion of the activated protein-1 transcription factor JunD induces oxidative stress and accelerates age-related endothelial dysfunction. Circulation 127:1229-40, e1-21 (2013). Read more (PubMed: 23410942) »

See 1 Publication for this product

Product Wall

I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the ce...

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En cuanto al principio de la reacción, en el “enzyme mix” no hay enzima XO. Este reactivo actúa en el segundo paso de la reacción, después de que la enzima XO de las muestras haya degradado la Xantina. El en...

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The cell pellet after removing all media can be stored at -80C with a small volume of PBS. It is not ideal to store cells with medium. Once thawed, the cells can be washed once with PBS and then the PBS can be removed. At this step the assay buffer can...

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I have double checked with the lab. Yes, the assay buffer is sufficient for efficient homogenization. You don’t need any other buffer. Just look at an aliquot of the homogenised sample under the microscope. If most cells show a broken nuclear ri...

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Thank you for your enquiry.

I am pleased to provide the following information for you:

1. Is there a preferred method to lift up the cells before centrifuging to get the supernatant/xanthine oxidase?

Cells can be trypsi...

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Thanks for your inquiry.
You can most likely use frozen tissues with limitations. We ourselves have never tried it, but I think if the tissues have been frozen efficiently and stored well, they should not pose a problem. Unfortunately we cannot gu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"