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Xanthine Oxidase Assay Kit (ab102522)

Q&A (2)

Overview

  • Product nameXanthine Oxidase Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 10 mU/ml
  • Range
    10 mU/ml - 1000 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Xanthine Oxidase Assay Kit is an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceXanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
  • Cellular localizationCytoplasm. Peroxisome. Secreted.
    • Alternative names
        Xanthine dehydrogenase/oxidaseXanthine oxidoreductaseXOXOR

    Applications

    Our Abpromise guarantee covers the use of ab102522 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Xanthine Oxidase Assay Kit images

    • Functional Studies - Xanthine Oxidase Assay Kit (ab102522)
      Functional Studies - Xanthine Oxidase Assay Kit (ab102522)
      H2O2 Colorimetric Standard Curve
    • Functional Studies - Xanthine Oxidase Assay Kit (ab102522)
      Functional Studies - Xanthine Oxidase Assay Kit (ab102522)
      Xanthine Oxidase Positive Control

    Protocols

    References for Xanthine Oxidase Assay Kit (ab102522)

    ab102522 has not yet been referenced specifically in any publications.

    Publishing research using ab102522 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Sir or Madam,

    I recently purchased the xanthine oxidase assay kit (ab102522) and just had a few questions about the protocol. For my assay, I am planning on measuring the enzyme activity of cultured cells in a P60 plate. However, it appear...

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    Thank you for your enquiry.

    I am pleased to provide the following information for you:

    1. Is there a preferred method to lift up the cells before centrifuging to get the supernatant/xanthine oxidase?

    Cells can be trypsiniz...

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    Hi, I am interested in your kit to measure xanthine oxidase in liver and plasma. Do you have any reports showing if it is duiable to use frozen tissue with this kit or is it absolutely necessary to run the experiment on fresh tissue?

    Thanks for your inquiry.
    You can most likely use frozen tissues with limitations. We ourselves have never tried it, but I think if the tissues have been frozen efficiently and stored well, they should not pose a problem. Unfortunately we cannot guara...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"