You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Xanthine Oxidase antibody (HRP) (ab34617)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-Xanthine Oxidase antibody (HRP)
See all Xanthine Oxidase products (6) ...

Description

Rabbit polyclonal to Xanthine Oxidase (HRP)

Conjugation

HRP

Tested applications

WB, ELISA, Immunomicroscopy, Dot Blotmore details

Cross reactivity

Reacts with

Mouse, Cow

Immunogen

Xanthine Oxidase (from Bovine Buttermilk)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography.

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Dot: Use at an assay dependent dilution.
ELISA: 1/35,000 - 1/175,000. Assayed against 1.0 ug of Xanthine Oxidase (Bovine
Buttermilk) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 146 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

Tissue specificity

Detected in milk (at protein level).

Involvement in disease

Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.

Sequence similarities

Belongs to the xanthine dehydrogenase family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 FAD-binding PCMH-type domain.

Post-translational
modifications

Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Cellular localization

Cytoplasm. Peroxisome. Secreted.

Target information above from: UniProt accessionP47989 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Xanthine dehydrogenase antibody
  • Xanthine dehydrogenase/oxidase antibody
  • Xanthine oxidase antibody
  • Xanthine oxidoreductase antibody
  • XD antibody
  • xdh antibody
  • XDH_HUMAN antibody
  • xdha antibody
  • XO antibody
  • xor antibody
see all

Database links

References for Anti-Xanthine Oxidase antibody (HRP) (ab34617)

ab34617 has not yet been referenced specifically in any publications.

Publishing research using ab34617? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"