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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-Xrn1 antibody
Description
Rabbit polyclonal to Xrn1
Tested applications
WB, IP, EMSAmore details
Cross reactivity
Reacts with
Human
Immunogen
A region between residue 1650 and the C-terminus (residue 1706) of human Xrn1 (SwissProt Q6MZS8)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/15000.Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
IP: Use at 2-5 µg/mg of lysate.
EMSA: Use at an assay dependent dilution. (PubMed: 20691152)
Target
Relevance
Xrn1 is the major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). May act as a tumor suppressor protein in osteogenic sarcoma (OGS). Associates with alpha and beta tubulins (By similarity). Found in a mRNP complex with RENT1, RENT2, RENT3B and XRN1.
Cellular localization
Cytoplasmic
Alternative names
- Dhm2 antibody
- mXRN1 antibody
- SEP1 antibody
- Strand-exchange protein 1 homolog antibody
Database links
- Entrez Gene: 54464 Human
- Omim: 607994 Human
- SwissProt: Q8IZH2 Human
Anti-Xrn1 antibody images:
Western blot - Xrn1 antibody (ab70259)
All lanes : Anti-Xrn1 antibody (ab70259) at 1/10000 dilution
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 194 kDa
Observed band size : 194 kDa
Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Immunoprecipitation - Xrn1 antibody (ab70259)
1 mg of whole cell lysate from HeLa cells was immunoprecipitated with ab70259 at 3ug/mg lysate (lane 1) or with normal rabbit IgG (lane 2). The subsequent blot was performed using ab70259 at 1ug/ml.
References for Anti-Xrn1 antibody (ab70259)
This product has been referenced in:
- Kim CWet al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA; Human.Read more (PubMed: 20691152) »
See 1 publication for this product
Publishing research using ab70259? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"