|
|
|
|
Western blot - Xrn1 antibody (ab70259)
(enlarge)
|
|
Immunoprecipitation - Xrn1 antibody (ab70259)
(enlarge)
|
|
|
|
|
Product Name
|
Xrn1 antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Xrn1
|
|
Immunogen
|
A region between residue 1650 and the C-terminus (residue 1706) of human Xrn1 (SwissProt Q6MZS8)
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/5000 - 1/15000. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
|
|
Relevance
|
Xrn1 is the major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). May act as a tumor suppressor protein in osteogenic sarcoma (OGS). Associates with alpha and beta tubulins (By similarity). Found in a mRNP complex with RENT1, RENT2, RENT3B and XRN1.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7 to 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Xrn1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Xrn1 antibody images, references, products related to ab70259 and other tools.
|
| |
Xrn1 antibody images:
|
 Western blot - Xrn1 antibody (ab70259)
All lanes : Xrn1 antibody (ab70259) at 1/10000 dilution
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 194 kDa
Observed band size : 194 kDa
Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
|
| |
Immunoprecipitation - Xrn1 antibody (ab70259)
1 mg of whole cell lysate from HeLa cells was immunoprecipitated with ab70259 at 3ug/mg lysate (lane 1) or with normal rabbit IgG (lane 2). The subsequent blot was performed using ab70259 at 1ug/ml.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Xrn1
|
| |
Xrn1 antibody - more information
|
Customer reviews (feedback) regarding Xrn1 antibody |
Customer FAQs regarding Xrn1 antibody |
Protocols for Xrn1 antibody |
Price and availability of products related to Xrn1 antibody |
| |
Xrn1 antibody - related products:
|
|
|
| |
Xrn1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Xrn1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
